Journal: Scientific Reports

Article Title: Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors

doi: 10.1038/srep14213

Figure Lengend Snippet: Data were normalized and the scale is indicated below the figures. ( a ) Unsupervised hierarchical clustering was performed for individual technical replicates. ( b ) Competitive inhibition of EV interactions with lectin microarray with mannose (Man) and N -acetylglucosamine (GlcNAc). ( c ) Lectin microarray of PNGase F-treated EVs. Significance (* = p ≤ 0.05) was determined by the ANOVA test.

Article Snippet: Probes (neoglyconjugates, glycoproteins and lectins) were printed on Nexterion® Slide H (Schott, Mainz, Germany) using a SciFlexArrayer S3 piezoelectric microarray printer (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C essentially as previously described .

Techniques: Inhibition, Microarray

Journal: bioRxiv

Article Title: A Photonic Biosensor-Integrated Tissue Chip Platform for Real-Time Sensing of Lung Epithelial Inflammatory Markers

doi: 10.1101/2022.06.21.497028

Figure Lengend Snippet: a) Design in GDS (Graphic Design System) format used to produce photolithographic masks for the photonic chip. The overall footprint is 5.8 × 4.0 mm, with 2 banks of 7 × 2 ring resonators, each with a total diameter of 300 μm including the trench (open area in the top layer of SiO 2 allowing direct contact between the ring resonator and analyte-containing solution). One temperature control ring (under oxide) is also provided in each bank. b) A photograph of the chip after fabrication and dicing. c) Functionalization chemistry using a succinic anhydride-bearing silane. d) Photomicrograph of a ring bank, with the antibody layout superimposed. e) An image acquired by the microarrayer immediately after spotting shows reproducible spotting of antibody solutions and StabilGuard over the rings. The dark spots are ∼250 pL drops settled in the ring trenches.

Article Snippet: After silanization, antibody solutions were printed on the rings using a Scienion SX SciFLEXarrayer piezoelectric microarrayer equipped with a PDC-60 capillary nozzle.

Techniques: Control

Journal: Microbiology Spectrum

Article Title: Nanobody engineering for SARS-CoV-2 neutralization and detection

doi: 10.1128/spectrum.04199-22

Figure Lengend Snippet: Relative binding strengths of trimeric nanobodies to RBD and Spike S1 domain variants on an antigen microarray. ( A ) Three multimodular nanobody constructs were tested for binding to RBD variants with amino acid changes K417N, E484K, or N501Y, and to spike the S1 domain with four amino acid changes (K417N, E484K, N501Y, and D614G). Fluorescence signals of Dylight 633-labeled multimodular nanobodies Tri-Ty1, Tri-TMH, and Tri-TMV bound to the different RBD and S1 variants are shown normalized relative to the signal from binding to wild-type RBD or S1 for each nanobody, respectively. Error bars represent the standard deviation of two replicate measurements. ( B ) Location of the amino acid changes within the SARS-CoV-2 RBD. Amino acid change N501Y is found in the Alpha and Beta variants ( , ), and the Beta variant displays the additional changes K417N and E484K . Delta variant carries none of the three tested RBD amino acid changes, while Omicron displays N501Y, K417N, and an alternative change at residue 484, E484A.

Article Snippet: Wild-type and variant SARS-CoV-2 RBD and spike S1 domains were biotinylated and arrayed as duplicate spots (0.1 ng per spot) in the wells of streptavidin-coated microtitration plates using a piezoelectric non-contact microarray dispenser (Nano-Plotter, GeSiM, Germany).

Techniques: Binding Assay, Microarray, Construct, Fluorescence, Labeling, Standard Deviation, Variant Assay, Residue